SOP FOR COMPOUND MICROSCOPE
- Ensure the working area is neat and clean.
- Place the compound microscope on the level surface.
- Connect the power cable of the compound microscope to the power source.
- Switch on the instrument.
- The LED indicator glows on the instrument indicating the instrument is ready for use.
- Set the required brightness through the brightness control knob.
- Position the slide on the stage with the sample to be observed on the upper surface of the slide.
- Adjust the slide to bring into the exact center of the light source.
- Low magnification objective lens shall be focussed first followed by a high magnification lens on the center of the sample.
- Turn the coarse adjustment knob to adjust the stage.
- While observing through the ocular bring the object into focus by adjusting with the coarse knob by using fine adjustment knob focus the sample for better resolution.
- Adjust the diaphragm lever to reduce or increase the light intensity to produce a clear and sharp image.
- Once the image is visible, adjust the slide for the targeted item in the image.
- To proceed from low power to high power magnification, rotate the high-power objective into position.
- Make a minor adjustment with the fine adjustment knob to sharpen up the image, if necessary.
- Whenever the objective is to be observed under oil immersion, place one-drop immersion oil or require quantity after it is focused with magnification. Then bring the oil immersion lens (100x) into position.
- Cover the instrument with the dust cover after use.
- Cover open types with dust protection caps.
- Remove dust and dirt from the visible optical surface using a brush or a cotton cloth.
- Remove water-soluble dirt with a dust-free cotton cloth.
- Store particularly sensitive components and accessories, such as objectives and eyepieces in a dry closet.
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